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5-Aminolevulinic acid photoactivated over planktonic and biofilm forms of Enterococcus faecalis as a pharmacological therapy alternative BJPS
Beltran-Leal,Adriana; Muñoz-Ruiz,Abraham; Esparza-Villalpando,Vicente; Castro,Yolanda; Pozos-Guillen,Amaury; Flores,Héctor.
The purpose of the study was to evaluate the antibacterial effect of protoporphyrin IX (PpIX) generated by the exogenous administration of 5-aminolevulinic acid or δ-ALA and activated with an argon laser over a planktonic and biofilm of Enterococcus faecalis (E. faecalis) as a pharmacological therapy alternative. A planktonic strain of E. faecalis was cultured with a solution of ∂-ALA (40 µg/mL)-thioglycolate solution for 13 min, and a biofilm of E. faecalis was cultured in a δ-ALA (80 µg/mL)-thioglycolate solution for 13 min. Then, both were irradiated with an argon laser. Finally, the antibacterial effect was evaluated by counting the CFU in planktonic form, and a LIVE/DEAD viability cell test. The production and accumulation of PpIX from exogenously...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Photodynamic therapy; 5-aminolevulinic acid.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100572
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CONTENIDO ESTOMACAL DE CHEILODACTYLUS VARIEGATUS VALENCIENNES 1833, PINGUIPES CHILENSIS VALENCIENNES 1833 Y PROLATILUS JUGULARIS VALENCIENNES 1833 EN BAHIA DE LA HERRADURA, COQUIMBO, DURANTE PRIMAVERA DEL 2001 Gayana
Moreno,Mabel; Flores,Héctor.
Se estudió el contenido estomacal de tres especies de peces importantes en la pesquería artesanal y que están presentes en Bahía La Herradura de Coquimbo: Cheilodactylus variegatus, Pinguipes chilensis y Prolatilus jugularis. Durante octubre y noviembre del 2001 se capturaron 20 ejemplares por especie, determinándose la frecuencia numérica (FN), volumétrica (FV) y de ocurrencia (FO) para cada uno de los ítem-presa encontrados. Esta información fue analizada mediante el IIR y para determinar la existencia de sobreposición de nicho alimentario se empleó el índice de Morisita. Se observó que Ch. variegatus y P. chilensis presentan una alimentación preferentemente basada en crustáceos como Rhynchocinetes typus y que P. jugularis se alimenta principalmente de...
Tipo: Journal article Palavras-chave: Indice de Morisita; Ítem-presa; Nicho alimenticio.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382002000200017
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Evaluation of Decellularized Matrix and ß-Tricalcium Phosphate as Biomaterials for Bone Neoformation: In vivo Study International Journal of Morphology
Muñoz-Ruíz,Abraham; Silva-Benítez,Erika; Soto-Sainz,Eduardo; Cerda-Cristerna,Bernardino; Ortiz-Magdaleno,Mariné; Pozos-Guillen,Amaury; Flores,Héctor.
The aim of this study was to evaluate histologically the effect of two biomaterials, a biomaterial derived from porcine Urinary submucosa Bladder Matrix (UBM) and beta-TriCalcium Phosphate (ß-TCP), on bone defects. Twenty male New Zealand rabbits were used; the models were divided in two groups: the UBM group; the ß-TCP group, and a Negative Control (NC) group. Five-mm defects were created in the femur of each model and then the different biomaterials were set in place depending on each group. At 4 and 8 weeks, the animals in the models were sacrificed and samples of the defect site were collected to perform a Hematoxylin and Eosin stain (H&E). Histologically, ß-TCP group at 4 and 8 weeks presented neoformation of bone-like and cartilage-like tissue,...
Tipo: Journal article Palavras-chave: Urinary bladder matrix; Bone regeneration; Biomaterial; SS-tricalcium phosphate.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300003
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